Direct Exposure of Monolayers of Mammalian Cells to Airborne Pollutants in a Unique Culture System.
Abstract
A mammalian cell culture and exposure system has been developed that allows direct exposure of living cells to atmospheres containing pollutants such as oxidant gases and hydrocarbon vapors. The system is being used to detect cytotoxic effects of various airborne materials as well as their possible mutagenic effects. Oxidant gases include nitrogen dioxide and ozone; other materials include hydrazine, aircraft fuels, and known mutagens such as ethylmethane sulfonate and ethylene oxide. The goals of the project are to detect possible toxic effects of airborne materials and to determine whether synergistic effects may be produced in mixtures of materials found in the environment. Methods have been developed for the small-scale generation of atmospheres containing known mutagens and test materials including hydrazine and its derivatives and common aircraft fuels. Tests for mutagenic effects of gaseous nitrogen dioxide on cultures of hamster lung cells have been negative at concentrations up to 10 ppm in air. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1979
- Accession Number
- ADA074180
Entities
People
- G. Scott Samuelsen
- J. T. Taylor
- M. E. Witte
- Ronald E. Rasmussen
- T. Timothy Crocker
Organizations
- University of California, Irvine