The Interaction of Pseudomonas Toxins with Epithelial Cell Membranes; The Primary Step in the Pathogenetic Sequence of Cellular Intoxication.
Abstract
During the past year, we have found: (i) how the electrical characteristics of the apical membrane of the turtle bladder epithelium are changed after exposure to each of three extracellularly-added, ADPR transferase containing bacterial toxins (e.g., the proenzymatic form of Pseudomonas toxin A, Cholera enterotoxin and Diphtheria toxin); (ii) how to segregate the apical from the basal-lateral membranes, how to identify each kind of membrane by uniquely-located enzymatic markers (e.g., ouabain-sensitive ATPase and a DIDS-binding protein mark the basal-lateral membrane; while a catecholamine-sensitive adenylate cyclase, a cyclic nucleotide-sensitive phosphoprotein kinase, and a concanavalin A-binding glycoprotein mark the apical membrane); and (iv) In recent preliminary experiments, we have found that the transport-stimulating effect of cholera toxin is eliminated when this transport has been pre-stimulated with norepinephrine; and conversely, the stimulatory effect of norepinephrine cannot be evoked when the transport parameter has been pre-stimulated with cholera toxin.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 28, 1979
- Accession Number
- ADA074563
Entities
People
- W. A. Brodsky
Organizations
- Icahn School of Medicine at Mount Sinai