Characterization and the Effect of Drugs on Pyruvate Kinase of Trypanosoma brucei,
Abstract
The following characteristics of T. brucei pyruvate kinase (PK) were examined: pH optimum; temp. optimum; Km; Mg++ requirement; effect of heterotropic modifiers; inhibition by ATP and pyruvate. The effects of some trypanocidal agents on enzyme activity were also noted. T. brucei PK resembles the A isoenzyme in response to amino acids and is sensitive to ATP but insensitive to pyruvate. Inhibition of activity by Hydroxystilbamidine, Berenil (diminazene) and Isometamidium was from 35-50% of controls at 10 to the minus 7th power M. With this preparation inhibition by Antrycide (quinapyramine) and Tryparsamide ranged from 5-10% of controls at 10 to the minus 7th power M.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 1979
- Accession Number
- ADA079511
Entities
People
- Arthur C. Zahalsky
- Valerie M. Ruppert
Organizations
- Southern Illinois University Edwardsville