Enzymology of Plant Cell Wall Breakdown by Plant Pathogens.
Abstract
The enzymatic degradation and structure of higher plant primary cell walls were the subjects of study. The enzymes employed included endopectate lyase, endo-beta-1,4 galactanase, endo-beta-1,4 xylanase and alpha-L-arabinofuranosidase. Cell wall material was from suspension cell cultures of Phaseolus vulgaris L. (bean) and Oryza sativa L. (rice). Extensive investigation of cell walls isolated from suspension cell cultures revealed that walls from both the dicot and monocot sources were quite heterogeneous with respect to cell wall types, and thus unacceptable for the proposed studies. Bean (dicot) cell walls proved to be much more susceptible to degradation by endopectate lyase than rice (monocot) cell walls. Other studies on the enzymolysis of these walls by other enzymes were not undertaken because they were considered to be too heterogeneous for the proposed studies. Preliminary investigation of other sources of primary cell wall revealed that tobacco pith cells represent a good source. Procedures were developed for the purification of an alpha-L-arabinofuranosidase from Sclerotinia sclerotiorum. The endopectate lyase produced by Erwinia chrysanthemi was shown to be inducible and subject to catabolite repression.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 1979
- Accession Number
- ADA080079
Entities
People
- Durward F. Bateman
- James R. Aist