Development of Short Term Immunotoxicological Assays for the Prediction of Chronic Toxicological Response Induced by Environmental Chemicals.
Abstract
The overall goal of the research is to systematically develop a battery of in vitro physical, biochemical, and functional assays to predict subchronic or chronic toxicologic behavior that would be produced by in nivo exposure to a chemical. The general experimental plan utilizes both in vivo and in vitro approaches. Results of in vivo tests are used as a reference to see if the in vitro tests can predict toxicologic responses. In vivo, mice were exposed subchronically to the environmental chemical trichloroethylene (TCE-2), and specific studies performed to assess bone marrow status, macrophage function, cell mediated immunity, humoral immunity, and standard toxicologic parameters. In vitro, a tier assay system is being developed, including two cytotoxicty tests, assessment of DNA synthesis, phagocytosis, lymphocyte responsiveness to mitogens, enumeration of bone marrow stem cells, and an antibody-forming cell assay (the Mischell-Dutton assay). The LD50's for male and female mice exposed by the gastrointestinal tract to TCE-2 were 2402 and 2454 mg/kg, respectively. A combination range-finding study of TCE-2 in the drinking water and 30-day interaction with emulphor (1%), there were only four parameters significantly different from the appropriate control, but none of these parameters showed dose dependency and could not be specifically attributed to an interaction between TCE-2 and emulphor.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1979
- Accession Number
- ADA080636
Entities
People
- Albert E. Munson
Organizations
- Virginia Commonwealth University School of Medicine