Recovery from Endotoxin Shock after Extracorporeal Perfusion without anticoagulation.
Abstract
The purpose of this study was to determine the effect of an extracorporeal nonanticoagulated perfusion system on survival from endotoxin shock in anesthetized closed-chest dogs. Dogs weighin approximately 18 kilograms were perfused 4 hours or served as nonperfused controls. In the perfused animals, blood was diverted from the distal aorta via plastic tubing at 1000 ml/min into a reservoir and returned by means of a roller-type pump to the femoral veins. Whole blood clotting times increased from a control of 7 minutes to greater than 24 hours within 45 minutes of perfusion in the absence of exogenous anticoagulation. After blood became incoagulable, animals were infused with 3 mg/kg E. coli endotoxin during a 30-minute period. Systemic pressures declined during the initial period but returned to baseline values, glucose remained at normal levels and all six dogs thus treated were healthy 7-day survivors. On the other hand, animals infused with endotoxin without extracorporeal perfusion demonstrated hypotension, hypoglycemia, and diarrhea, and five of six dogs died within 36 hours. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 27, 1979
- Accession Number
- ADA081177
Entities
People
- Beverly Beller-todd
- Lerner B. Hinshaw
- Linda T. Archer
Organizations
- University of Oklahoma Health Sciences Center