Kinetics of Neutrophil-Releasing Activity of Post-Leukopheresis Plasma.
Abstract
The present studies have focused on assessing factors in the animal model developed by us which might bear an interpretation of results, not only in the animal, but ultimately in the human donor. These include the effect of deep vs light anesthesia, anticoagulation, and interaction of the blood with plastic cannulae. The maintenance of deep anesthesia is necessary if one wishes to eliminate a triggering of granulocyte mobilization, probably via the pituitary/adrenal axis. It has shown also that blood/plastic interaction also contributes to granulocyte mobilization, and this must be considered in interpreting some of these experiments. It has demonstrated that homologous plasma does not significantly affect the granulocyte count while heterologous plasma does, perhaps as a result of foreign protein interactions. The gluococorticoid, prednisolone, was shown to mobilize granulocytes in the rat to a degree similar to that in humans but at a much more rapid rate. A mathematical model which gives a much more accurate measure of the magnitude of granulocyte mobilization has been constructed. This model will be invaluable not only to better understand the results obtained in these experiments but will also be useful for the study of granulocyte kinetics in general.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 15, 1980
- Accession Number
- ADA082033
Entities
People
- Albert J. Roy