Isolation and Characterization of Plasmodial and Babesial Antigens.
Abstract
The objective of this project was the isolation and characterization of antigens of plasmodia and babesia. Part II is a critical review of procedures for the isolation of malarial parasites and for their fractionation. This review provides an analysis of the problem confronting those attempting to isolate antigenic materials from plasmodia. Parts III and IV are descriptions of research on the mechanisms by which the host resists plasmodial infection and by which the plasmodium defends itself from the host's response. These studies show clearly the importance of the merozoite capsule in the resistance of the blood inhabiting stages of plasmodia to the immune responses of the host. They also define the role of antibody and macrophages in the host's response to plasmodial infection. The final section, Part IV of this report, describes the isolation and characterization of a soluble component of Plasmodium berghei which induces immunity to challenge in rats. This component is obtained by gentle washing of free parasites and may be capsular material. It is proteinaceous and was shown by disk gel electrophoresis to contain only a small subset (2-4) of the proteins present in the parasites.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 10, 1979
- Accession Number
- ADA082217
Entities
People
- Julius P. Kreier
Organizations
- Ohio State University