Status Report on Cryopreservation of Human Platelets.
Abstract
The equivalent of about 5 single units of platelets was isolated from each of eight normal volunteers by intermittent flow centrifugation using the Haemonetics Model 30 Blood Processor. The platelets were frozen with 6% DMSO in a single polyolefin plastic container in a -80 C mechanical freezer. In vitro freeze-thaw-wash platelet recovery values varied with the methods of measurement: 80% when the platelet counts were made by phase microscopy, 85% by Coulter counter, and 60% by the Technicon. These platelets had 51Cr survival values in vivo about 50% of those observed for fresh autologous platelets. In another study, platelets isolated from a unit of blood by serial differential centrifugation were frozen in inexpensive polyvinyl chloride plastic bags and in special expensive polyolefin plastic bags: in vitro recovery and in vivo survival values were similar in the two groups. Platelets frozen in the polyolefin plastic bags released 14C-serotonin at increasing concentrations of thrombin at about the same capacity as fresh platelets; the response of platelets frozen in polyvinyl chloride plastic bags was about 90% that of platelets frozen in polyolefin plastic bags. We are now isolating multiple units of platelets by intermittent flow and by continuous flow centrifugation and freezing them in polyvinyl chloride transfer packs to support thrombocytopenic patients. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 27, 1979
- Accession Number
- ADA082336
Entities
People
- C. Robert Valeri
- John J. Vecchione
Organizations
- Boston University