The Origin of Cataracts in the Lens from Infrared Laser Radiation.
Abstract
Changes in lens constituents, including proteins, following exposure to laser radiation have been used to detect the earliest changes possible in cataract formation. The results of current experiments are described. Calf, rabbit, rat, pig, and human lenses were incubated at various temperatures between 40-45 C. Purified lens crystallins were also incubated at temperatures from 40-45 C to determine the contribution of thermal elevation to denaturation possibly leading to cataractogenesis. Thin film gel electrophoresis, column chromatography, electron probe x-ray microanalysis, and amino acid separation were the analytical methods used on the incubated lenses, as well as lenses from rabbit eyes exposed to a 1.06 micrometers CW neodymium laser in vivo. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 20, 1977
- Accession Number
- ADA084055
Entities
People
- B. S. Yamanashi
- M. A. Orr
- M. L. Wolbarsht
Organizations
- Duke University