Rickettsial Diseases: Scrub Typhus and Trench Fever.
Abstract
This study was directed towards the isolation of rickettsial antigens and detection of antibodies. Stock supplies of Gilliam, Kato and Karp strains of Rickettsia tsutsugamushi were propagated in embryonated chick eggs and used for infecting cultured cells. Infected McCoy cell extracts containing soluble anti-gen as well as infected yolk sacs were inoculated into guinea pigs to produce antisera. However, the yield of antibody was low and only detectable by fluorescent antibody techniques. More recently, progress was made in growing R. tsutsugamushi in BHK sub 21 cells as a monolayer or in suspension cultures. Irradiated cells seem to enhance rapid rickettsial proliferation Solubilization after freezing and thawing and by sonication and Triton X-11 treatment was used to isolate soluble antigen for characterization and further purification. In these studies, infected cells as well as isolated samples used in the studies were fixed and examined by electron microscopy to monitor the structure of the organism and the host cell as well as some of the isolated and purified samples.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1979
- Accession Number
- ADA085856
Entities
People
- Susumu Ito
Organizations
- Harvard T.H. Chan School of Public Health