Immobilized Enzymes for Automated Analyses.
Abstract
Methods were developed for analyzing enzyme immobilization processes applicable to carrier materials suitable for automated clinical chemistry analyzers. Immobilization was analyzed in terms of (a) amount of bound protein, (b) surface activity, (c) quantity of activating surface chemical groups on a carrier surface, and (d) total available carrier surface area. Carriers were utilized in the form of microfibers or microspheres so as to present very large specific surface areas for immobilization, 39,000 to 150,000 sq cm/g of carrier. The methods were applied to study of immobilization of glycerol dehydrogenase (GD). Three distinct types of immobilization processes were analyzed: (a) irreversible physical adsorption to nylon 6,6; (b) adsorption to charged amine-glass; and (c) dialdehyde-linked covalent coupling to amine-glass. Adsorption to nylon (process a) indicated the importance of nicotinamide adenine dinucleotide (NAD) during immobilization.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1979
- Accession Number
- ADA087339
Entities
People
- George Herzlinger
- Jean Thompson