Purification of Transketolase from Human Erythrocytes. 1. Using Solvent Denaturation as the Initial Step.
Abstract
An attempt was made to purify human erythrocyte transketolase (EC 2.2.1.1) by the use of solvent denaturation as the initial isolation step. This involved the use of 95% ethanol solution (-20 C) to denature a large amount of the hemoglobin and other non-transketolase proteins. Although the data obtained from this study did not contribute to sound statistical values because of the small sample size, they tended to support the application of the method as an effective initial step in the purification of human erythrocyte transketolase. The mean recovery of the enzyme for five trials conducted with blood obtained from three donors was 61 + or - 3%. The purification ranged from 21 to 23. The method permitted the removal of nearly 95% of the hemoglobin initially present. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1980
- Accession Number
- ADA088257
Entities
People
- Evelyn L. Mcgown
- Howerde E. Sauberlich
- James H. Skala
- Michael H. Dong
- Paul P. Waring