Studies on PGBX: Fractionation by a Combination of Dialysis and Molecular Exclusion Chromatography.

Abstract

By combining the techniques of dialysis and molecular exclusion chromatography (MEC), a Type III prostaglandin PGBX preparation could be separated into fractions containing different polymer composition and different amounts of in vitro PGBX activity. An over-all purification of PGBX activity was obtained in fraction 3 that contained 20% of the total weight and 40% of the total PGBX activity with a 2 fold purification of specific activity of the PGBX inhibitor factor. This fraction, which was retained by the membrane on aqueous dialysis, and not retained by the membrane on methanol dialysis, had a long retention time on MEC, and gave molecular weight (vapor pressure osmometry) of 1789. Furthermore this fraction was homogeneous by analytical MEC. (Author)

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Document Details

Document Type
Technical Report
Publication Date
Aug 22, 1980
Accession Number
ADA089417

Entities

People

  • E. Soffer
  • H. W. Shmukler
  • M. G. Zawryt
  • S. F. Kwong
  • W. Feely

Organizations

  • Naval Air Warfare Center Warminster

Tags

Communities of Interest

  • Biomedical
  • Sensors

DTIC Thesaurus Topics

  • Absorption
  • Absorption Spectra
  • Albumins
  • Alcohols
  • Biological Sciences
  • Calibration
  • Chemistry
  • Chromatography
  • Detectors
  • Dialysis
  • Dielectric Polymers
  • Flow Rate
  • Health Services
  • Mitochondria
  • Molecular Weight
  • Standards
  • Vapor Pressure

Fields of Study

  • Chemistry

Readers

  • Mathematics or Statistics
  • Organic Chemistry
  • Solar Photovoltaics and Thermoelectric Devices.