Schistosome Materials for Vaccine Development.
Abstract
A Puerto Rican strain of Schistosoma mansoni was maintained in Biomphalaria glabrata snails and Swiss albino mice. Uninfected snails were raised in six 20-gallon aquaria. Approximately 200 snails (5-7mm dia.) were collected each week and exposed individually with 6-8 miracidia. When requested, snails were exposed to 1 miracidium each for development of single-sex schistosomal infections. Miracidia were derived from livers of 8-week infected mice. A constant supply of approximately 400 infected snails was maintained for production of cercariae. Weekly yields of from 1 to 3 million cercariae were processed as needed or used for experimental work. Adult parasites were perfused from 30 mice per week and used for production of adult worm antigens. Due to reduction in funds, compared to previous years, no schistosome eggs, schistosomules, or cercarial secretion enzymes were collected on a regular basis for investigators at NMRI. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 24, 1980
- Accession Number
- ADA089941
Entities
People
- F. A. Lewis
- M. A. Stirewalt
Organizations
- Instituto de Investigaciones Biomédicas