Mechanism of Innate Resistance to Viral Encephalitis.
Abstract
Factors that influence the phenotypic expression of genetic resistance or susceptibility to lethal flavivirus infection were investigated in 2 congenic strains of mice: C3H/RV (resistant to lethal infection) and C3H/He (susceptible to lethal infection). C3H/He and C3H/RV mice were equally susceptible to infection by Banzi virus when inoculated intraperitoneally (IP). Encephalitis developed in both strains after IP inoculation and mortality was high in C3H/He mice whereas mortality was low in C3H/RV mice. The difference in susceptibility between the 2 strains, based on mortality, ranged from 10 to the 4th power to 10 to the 6th power LD sub 50. Resistance of C3H/RV mice to lethal encephalitis developed postnatally and did not reach significant levels until mice were 4 weeks old. Resistance was abrogated by cyclophosphamide-treatment, sublethal x-irradiation (400R) and T-cell depletion. Virus yields, distribution of viral antigen and lesions in brain of immunologically crippled C3H/RV mice closely resembled those in untreated C3H/He mice. Infected, cyclophosphamide-treated C3H/RV mice also developed severe pancreatitis. Intracerebral (IC) inoculation of Banzi virus produced high mortality in both strains and virus titers and lesions in brains were also similar.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1976
- Accession Number
- ADA090468
Entities
People
- Pravin N. Bhatt
- Robert O. Jacoby
Organizations
- Yale University