Plasmid Isolation in Legionella pneumophila and Legionella-like Organisms.
Abstract
Agarose gel electrophoresis was employed to screen nine strains of Legionella-like bacteria and one strain of Legionella pneumophila for the presence of extrachromosomal deoxyribonucleic acid. Cryptic plasmids were found in three of the isolates examined. A purification protocol was devised which consolidates the central tenets of several existing isolation techniques into a single procedure. Plasmid molecular weights were determined to range from 46.6 to 59.8 megadaltons (Mdal). (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 22, 1980
- Accession Number
- ADA090844
Entities
People
- Gregory B. Knudson
- J. W. Ezzell
- Perry Mikesell
Organizations
- United States Army Medical Research Institute of Infectious Diseases