Influence of Cycloheximide and 1,25-Dihydroxyvitamin D3 on Mitochondrial and Vesicle Mineralization in the Intestine

Abstract

1,25(OH)2D3 increases cell permeability to calcium. This increase is not mediated by proteins sensitive to cycloheximide or actinomycin D inhibition. We propose that CaBP may associate with intracellular membranes and organelles to prevent intracellular calcium accumulation and the potential cytotoxic effects of such accumulation. In support of this hypothesis, the amount of mitochondrial mineralization in chick intestinal cells was markedly increased by 1,25(OH)2D3 treatment when CaBP synthesis was simultaneously blocked by cycloheximide treatment. Mineral in membrane vesicles was increased by 1,25(OH)2D3 treatment, but was blocked by simultaneous treatment with cycloheximide.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1980
Accession Number
ADA091396

Entities

People

  • Daniel D Bikle
  • David T. Zolock
  • Paul W. Mellick
  • Robert L. Morrissey

Organizations

  • Letterman Army Hospital

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Cell Membrane
  • Cells
  • Cellular Structures
  • Electron Microscopes
  • Electron Microscopy
  • Electrons
  • Epithelial Cells
  • Laboratory Animals
  • Membranes
  • Microscopes
  • Mineralization
  • Mitochondria
  • Observation
  • Organelles
  • Permeability
  • Precipitates
  • Vitamin D

Fields of Study

  • Biology

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