Identification of Leukocyte Subpopulations Responsible for the Production of Osteoclast Activating Factor and their Role in Bone Resorption.
Abstract
Osteoclast Activating Factor (OAF) was predictably produced in large volume mitogen stimulated MNC cultures. OAF production was independent of DNA synthesis. OAF was produced in mixed leukocyte cultures containing heat inactivated fetal calf serum. OAF production was independent of blastogenesis. OAF activity was isolated in the molecular weight range of 12,000 to 25,000 daltons by S-200 Sephacryl chromatography and high performance liquid chromatography (HPLC). OAF activity was concentrated across membranes and by ammonium sulfate precipitation. Subsequent HPLC produced two peaks of bone resorbing activity: a major peak at 18,000 daltons and a minor peak at 9,000 daltons. MNC culture supernatants also contained high concentrations of low molecular weight prostaglandins which also are believed to be osteolytic in vitro. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 31, 1980
- Accession Number
- ADA093782
Entities
People
- Charles E. Hawley
Organizations
- United States Army Institute of Surgical Research