Changes in Tissue Cyclic AMP Concentrations following an Intravenous Lethal Dose of Cholera Enterotoxin in Rabbits,
Abstract
The purpose of this study was to test the hypothesis that a generalized increase in tissue cAMP concentrations after an IV injection of cholera enterotoxin may play a role in the development of biochemical and pathological changes leading to death. Dutch rabbits allocated as control (n equals 9) and challenged (n equals 13) groups were injected IV with highly purified cholera enterotoxin at a dose of 100 or 200 micrograms/kg. When death appeared imminent (20-30 hr after inoculation), rabbits were anesthetized; plasma urine and 13 different tissue samples were obtained for cAMP determinations. When results from toxin-injection rabbits (100 micrograms/kg) were compared with those of controls, no significant changes were observed in any tissue studied. A higher dose of enterotoxin (200 micrograms/kg) produced significant increases in cAMP concentrations in lung, spinal cord, skeletal muscle, renal cortex, renal medulla and liver. The elevation of cAMP values in selected tissues suggests differences in tissue sensitivity to cholera enterotoxin; the data fail to support in toto the proposed hypothesis. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 13, 1981
- Accession Number
- ADA094352
Entities
People
- C. T. Liu
- E. J. Galloway
Organizations
- United States Army Medical Research Institute of Infectious Diseases