Safety and Immunogenicity Testing of a Pilot Polysaccharide Vaccine Preparation to Pseudomonas aeruginosa.
Abstract
Purification techniques were developed for obtaining a high molecular weight polysaccharide preparation for testing as a vaccine to infections caused by the bacterium Pseudomonas aeruginosa. A pilot preparation from an immunotype 7 strain of this organism was made after consideration of using anion exchange resins and colonial morphology criteria for selecting techniques used for purification. Serological assays in the form of a radioactive antigen binding assay and a splenic plaque forming cell assay were developed to assay the polysaccharide preparations for immunogenicity. Finally testing of the polysaccharide preparations in animal models of P. aeruginosa infection have commenced. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1979
- Accession Number
- ADA095135
Entities
People
- Gerald B. Pier