Affinity Chromatographic Purification of Endogenous Pyrogen with Cibacron Blue.

Abstract

Human and rabbit endogenous pyrogens bind to affinity columns of both Cibacron Blue agarose and its carboxymethyl derivative. All pyrogenic activity from both sources is recovered by eluting with a high salt concentration buffer. This affinity chromatographic step purified crude rabbit endogenous pyrogen 5-fold (from 118 to 588 rat pyrogen doses/mg) or gel filtered rabbit pyrogen 10-fold (up to 5,820 rat pyrogen doses/mg). (Author)

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Document Details

Document Type
Technical Report
Publication Date
Jan 14, 1981
Accession Number
ADA095970

Entities

People

  • Berthony Ladouceur
  • Charles F. Matson
  • Edward C. Hauer
  • Walter J. Critz

Organizations

  • United States Army Medical Research Institute of Infectious Diseases

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Acetic Acid
  • Albumins
  • Animals
  • Assays
  • Biomedical Research
  • Cells
  • Chromatography
  • Diseases And Disorders
  • Filtration
  • Infectious Diseases
  • Laboratory Animals
  • Maryland
  • Materials
  • Molecular Weight
  • Molecules
  • Polysaccharides
  • Proteins

Fields of Study

  • Biology

Readers

  • Analytical Chemistry
  • Immunology
  • Molecular Genetics