Genetics of Novel Hybrid Bacteriophage and Development of Generalized Transducing System for Salmonella typhosa.
Abstract
Hybrids phages between Salmonella Phage P22 and coliphage and Phi80 have been isolated by using E. coli-S. typhimurium hybrids. Among those hybrid phages, phi80immP22dis hybrids carrying both immunity related genes, c and Im genes of P22 were isolated. Since P22 tail component gene 9 and somatic antigen conversion gene al are located between the c and Im genes of P22, we examined whether phi80immP22dis hybrids carry these genes. Some Phi80immP22dis hybrids carry gene al but not gene 9 whereas the remaining Phi80immP22dis hybrids carry gene 9 only. No Phi80immP22 hybrid phages containing both the P22 genes 9 and al were found. These observations suggest that Phi80immP22dis hybrid is formed as a consequence of multiple crossovers. Although lambda immP22dis hybrid phages carry both genes 9 and al, Phi80immP22dis hybrids carry only one of these genes 9 or al. Since the size of Phi80 phage genome is about 92% of the lambda genome, we concluded that the Phi80immP22 gene is unable to contain both genes 9 and al.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1979
- Accession Number
- ADA100360
Entities
People
- Nobuto Yamamoto
Organizations
- Temple University