Fluorometric Method for Determination of Uric Acid in Flour

Abstract

A fluorescence technique is described for the determination of uric acid content in flour and its use as an index of insect infestation. Uric acid, a major component of the excreta of adult Tribolium used in this study for natural infestation, accumulates in infested products providing a quantitative measure of past plus present degree of infestation. The efficiency of uric acid extraction from flour with 0.2 M sodium acetate, checked by adding known amounts of uric acid to flour, is shown to be about 96-98% accurate. The method is capable of detection of uric acid at the level of 3.0 mg per 100 g of flour.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1980
Accession Number
ADA100657

Entities

People

  • L. G. Holmes
  • L. W. Smith Jr.

Organizations

  • United States Army Soldier Systems Center

Tags

DTIC Thesaurus Topics

  • Acids
  • Biomedical Research
  • Containers
  • Emission
  • Emission Spectra
  • Filter Paper
  • Filters
  • Fluorescence
  • Insects
  • Instrumentation
  • Materials
  • New York
  • Recovery
  • Spectra
  • Spectroscopy
  • Ultraviolet Spectroscopy
  • Uric Acid

Fields of Study

  • Biology

Readers

  • Analytical Chemistry
  • Vector-Borne Disease and Entomology