A New Instrument for Time-Resolved Reduction of Scattered Radiation in Fluorescence Measurements.
Abstract
An opto-electronic cross-correlation system was employed to reduce the scattering influence in fluorescence measurements. A stable optical delay line incorporated into the instrument was positioned to yield detection at a fixed time after excitation; the optimal delay time was determined simply from the ratio of the fluorescence decay curve to a similar curve portraying scattering response. Signal-to-scattering background enhancements greater than two were measured for the very short-lived (TAU-0.07 ns) fluorophores whereas a six-fold increase was measured for longer lifetime fluorophores. The shortest lifetime which would benefit from time-resolution in this system is limited by the time-response of the PMT (1.1 ns FWHM); the excitation pulses are on the order of 6 ps. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1981
- Accession Number
- ADA101133
Entities
People
- Gary M. Hieftje
- R. E. Russo
Organizations
- Indiana University