A Computer System for Combined Neuronal Mapping and Morphometry,
Abstract
A computer system has been developed which allows both mapping and planimetry of histological preparations. Data consist of cytoarchitectonic boundaries defined in low power micrographs of thick sections, and nuclear and vascular profiles defined in high power micrographs of thin sections taken from the original thick tissue, and landmarks common to both. Data from these different preparations and magnifications are entered into the computer by a digitizing tablet and are stored as points in a real-world coordinate system, along with profile labels, landmarks for alignment purposes, and section depth. Subsequent programs determine parameters such as area and perimeter for each profile, as well as its geometric center and relationship to the cytoarchitectonic boundary. This mapping parameter allows morphometric analysis of profiles as a function of their position. Other programs allow display and manipulation of data in three dimensions, cell counts and stereology. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1980
- Accession Number
- ADA101216
Entities
People
- C. A. Curcio
- K. R. Sloan Jr.
Organizations
- University of Rochester