Inhibition of Alkaline Phosphatase by Several Diuretics

Abstract

Acetazolamide, furosemide, ethacrynic acid and chlorothiazide, diuretics of considerable structural diversity, inhibit alkaline phosphatase. The inhibition is reversible and the mechanism is of the mixed type, having both competitive and non-competitive characteristics. K sub i is calculated to be 8.4, 7.0, 2.8 and 0.1 mmol/l for acetazolamide, furosemide, ethacrynic acid and chlorothiazide, respectively. Chlorothiazide is a much more potent inhibitor of alkaline phosphatase than the other three diuretics. The combination of ethacrynic acid and cysteine, itself an alkaline phosphatase inhibitor, is less inhibitory than ethacrynic acid alone. Rat and human kidney alkaline phosphatase are equally sensitive to chlorothiazide, ethacrynic acid and furosemide.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1980
Accession Number
ADA102778

Entities

People

  • Gary H. Price

Organizations

  • United States Army Institute of Surgical Research

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Acetazolamide
  • Amides
  • Anticonvulsants
  • Aqueous Solutions
  • Biochemistry
  • Cell Membrane
  • Chemistry
  • Connective Tissue
  • Cysteine
  • Diuretics
  • Enzymes
  • Inhibition
  • Inhibitors
  • Kidneys
  • Tissues
  • United States Military Academy

Readers

  • Cardiovascular Physiology
  • Underwater engineering and Marine Technology.