Effect of Chemical Mutagens on Herpes Virus - Induced Cellular Transformation and Testing for Mutagenesis in Mouse Cells

Abstract

The temperature sensitive (ts) mutant A8(293) of herpes simplex type 2 virus (HSV-2) was employed in tests to detect chemical interactions with the virus causing increased transformation of target cells. Mouse 3T3 cells were monitored for morphological transformation. Hydrazine and 1,2-dimethylhydrazine caused an enhancement of viral transformation of between three to four fold, confirming earlier findings using ultraviolet light inactivated wild type virus. Norharman (pH-pyrido(3,4-6)indole), a component to tobacco tars, caused a nearly three-fold enhancement of transformation. Xylene and toluene were not found to enhance viral transformation. Experiments involving host-mediated activation of the test chemicals were unsuccessful because of low target cell recovery due to inhibition of the target 3T3 cells.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 1981
Accession Number
ADA102854

Entities

People

  • F. B. Johnson

Organizations

  • Brigham Young University

Tags

Communities of Interest

  • Human Systems

DTIC Thesaurus Topics

  • Air Force
  • Chemical Compounds
  • Culture Techniques
  • Dimethylhydrazines
  • Ecotoxicology
  • Hydrazines
  • Infection
  • Inhibition
  • Mutagens
  • Organic Compounds
  • Scientific Research
  • Security
  • Time Intervals
  • Toxicity
  • Universities
  • Wound Infections

Fields of Study

  • Biology

Readers

  • Infectious Disease/Epidemiology
  • Molecular Genetics
  • Oncology (Cancer Research).