Radiometric Methods for Rapid Diagnosis of Viral Infection.
Abstract
A rapid radiometric technique was developed for detecting the presence of herpes simplex virus type 1 in stationary monolayers of the diploid cell line WI-38. The time of detection was compared to that obtained from visual examinations for cytopathic effects in the same cell line. Glucose-1-14C oxidation of infected and uninfected cells was determined by 14CO2 production by the ionization chamber, Bactec R-301. Infected cells showed a 23-26% reduction in glucose-1-14C oxidation 4-6 hours post-infection, as compared to uninfected control cells. This change in cellular metabolism was observed 14 hours before visible signs of cytopathic effect. Specific antiserum will be used to neutralize the viral effect for the purpose of speciation. The radiometric method for the detection of viral effect on host metabolism is simple, objective, and deserves further investigation. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 15, 1974
- Accession Number
- ADA103119
Entities
People
- Henry N. Wagner Jr
- Patricia Charache
- Steven M. Larson
Organizations
- Johns Hopkins University