Cytologic and Biochemical Genetic Effects of Chemical Carcinogens
Abstract
The first project is concerned with the relationship between initiation of cancer by chemicals and the damage and repair of DNA in cells and tissues susceptible to the carcinogenic action of the chemicals. The working hypothesis is that interaction of the carcinogen with cellular DNA is a necessary step in carcinogenesis and that this interaction may cause DNA damage which is then followed by DNA repair synthesis. The second project was conducted with the cooperation of the staff of the Toxic Hazards Research Unit at AMRL, WPAFB. Rats and hamsters were exposed by inhalation to hydrazine at 750 ppm for one hour. Groups of animals which had been exposed only once or which had been exposed 10 times were shipped to UC Irvine for study. The factors studied were (1) DNA replication in the lung; (2) DNA repair synthesis in the lung; (3) Cellular replication in the lung and nasal turbinates; and (4) Metabolism of a known lung carcinogen, 3H-benzo(a)pyrene, by lung slices from the treated animals. The third project was initiated during the current year with the goal of developing an animal model in which biochemical and cytogenetic factors could be studied during the process of tumorigenesis. Range-finding studies are underway at the Toxic Hazards Research Unit (THRU) to establish the intratracheal dosage of 3-methylcholanthrene (MCA) necessary to produce a high yield of lung tumors in Fischer 344 rats within 12 months.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1981
- Accession Number
- ADA107615
Entities
People
- Ronald E. Rasmussen
Organizations
- University of California, Irvine