The Role of Inducible DNA Repair in W-Reactivation and Related Phenomena.
Abstract
Most of the UV-induced photoproducts in the DNA of bacteria and their viruses are repaired by 'error-proof' repair mechanisms such as photoreactivation, ('short-patch' excision repair, and recombinational repair. Single-strand gaps resulting from replication of DNA, which contains pyrimidine dimers, can be repaired by ultraviolet (UV)-inducible, recA+ lex+-dependent 'error-prone' repair ('SOS' repair) which leads to mutations in the bacteria and its phages. Other UV-induced, recA+ lex+-dependent functions, such as prophage induction, filamentous growth, and W-reactivation, are coordinately regulated with the error-prone repair functions. An increasing amount of experimental evidence suggests that misincorporation of bases could result from an inducible factor which affects the proofreading activities of DNA polymerase, allowing it to fill postreplicative daughter strand gaps across from non-coding UV photoproducts. Mutagenic W-reactivation illustrates the role of UV-inducible, recA+ lex+-dependent functions in the error-prone DNA repair of bacteria and their viruses. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 14, 1981
- Accession Number
- ADA108048
Entities
People
- Gregory B. Knudson
Organizations
- United States Army Medical Research Institute of Infectious Diseases