A Turbido-Haemolytic Assay for Complement,
Abstract
A method for the haemolytic assay of complement is described. The method is based on the turbidimetric quantitation of sheep red blood cells at a wavelength of 640 nm. This approach allows for the continuous and simultaneous assessment of complement activity and results in a substantial reduction in the time required to perform complement assays as opposed to the conventional haemolytic assays. The turbido-haemolytic assay is used to investigate the anti-complementary activity of kappa and lambda carrageenans. Lambda carrageenan, the more sulphated polymer, is the more potent inhibitor of complement activation. The application of this technique to the detection and/or identification of microorganisms is presently being investigated. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 1981
- Accession Number
- ADA109914
Entities
People
- V. K. Chenier
- V. L. Di Ninno
Organizations
- Defence Research and Development Canada