Partial Characterization of Small Plasmid Deoxyribonucleic Acid Present in ' Neisseria Meningitidis',

Abstract

The presence of plasmid DNA in Neisseria meningitdidis was investigated. N. meningitidis isolates from clinical meningitis and highly virulent in mice, were found to harbour a small plasmid. Agarose gel electrophoresis of crude lysates from different strains revealed a single plasmid DNA species with the same apparent electrophoretic mobility. Using agarose gel electrophoresis its molecular weight was calculated as 3.4 megadaltons. Electron microscopic observation revealed tdhat plasmid DNA molecules were predominently in the covalently closed form. The estimated contour length of the open circular form was 1.6 micro. The function of the plasmid DNA is presently unknown. (Author)

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Document Details

Document Type
Technical Report
Publication Date
May 01, 1981
Accession Number
ADA109915

Entities

People

  • A. R. Bhatti
  • K. O'hara
  • L. A. White
  • L. E. Bryan

Organizations

  • Defence Research and Development Canada

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Acids
  • Antigens
  • Deoxyribonucleic Acids
  • Diseases And Disorders
  • Electron Microscopes
  • Electron Microscopy
  • Escherichia
  • Escherichia Coli
  • Gel Electrophoresis
  • Meningitis
  • Microscopes
  • Molecular Weight
  • Molecules
  • Nucleic Acids
  • Observation
  • Standards

Fields of Study

  • Biology

Readers

  • Immunology
  • Molecular Genetics

Technology Areas

  • Microelectronics