Freeze-Thaw Induced Damage to Endothelial Cells in Vitro. I. Development of a Suitable System,
Abstract
The study of freeze-thaw damage to endothelial cells necessitated the development of a suitable in vitro system. After investigating various substrates, Leighton tubes containing a plastic coverslip proved to be the most versatile method for studying alterations to endothelial cells following thaw. The polymethylpentene coverslip was the key to its versatility. It was easily cut which allowed portions of the same monolayer to be processed for examination by phase contrast, brightfield, and bth scanning and transmission electron microscopy. At the same time, the attached handle allowed easy manipulation without disturbing the monolayer. Also, following a freeze-thaw insult, endotheial cells remained better attached to the plastic substrate than to glass coverslips. The Leighton tube itself was ideal for temperature studies because it was water tight which allowed submersion in refrigerated water baths. The media covering the cells can be assayed for cellular enzymes released into the media. In this manner, biochemical markers from the same cells may be correlated with cellular structure. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 25, 1981
- Accession Number
- ADA111074
Entities
People
- Albert W. Guzman
- Carol J. Baker
- Lynn R. Trusal
Organizations
- United States Army Research Institute of Environmental Medicine