Mutagenicity Evaluation of Otto Fuel Number 2 in the Ames Salmonella/ Microsome Plate Test. Segment Report
Abstract
The test compound was examined for mutagenic activity in a series of in vitro microbial assays employing Salmonella and Saccharomyces indicator organisms. The compound was tested directly and in the presence of liver microsomal enzyme preparations from Aroclor-induced rats. The compound was tested over a series of concentrations such that there was either quantitative or qualitative evidence of some chemically-induced physiological effects at the high dose level. The low dose in all cases was below a concentration that demonstrated any toxic effect. The dose range employed for the evaluation of this compound was from 0.005 microliter to 10 microliter per plate. The compound was toxic to all the strains except TA-1538 and D4 at 5 microliter and 10 microliter per plate. Toxicity was also observed with the strain TA-1535 at 1 microliter per plate. The results of the tests conducted on the compound in the absence of a metabolic system were all negative. The results of the tests conducted on the compound in the presence of the rat liver activation system were all negative. The test was repeated with TA-100 at the three lowest doses and intermediate doses of 0.05 microliter and 0.5 microliter per plate because of the increase in the number of revertants observed in the initial test. The repeat tests were also negative.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 1979
- Accession Number
- ADA112227
Entities
People
- D. R. Jagannath