Standard Operating Procedure for Mutagenicity Testing Using the Drosophila melanogaster Sex-Linked Recessive Lethal Assay.
Abstract
The methodology and work schedule followed at Letterman Army Institute of Research of mutagenicity testing of selected materials using the Drosophila melanogaster sex-linked recessive lethal (SLRL) assay are described. Testing procedures were designed to insure compliance with the Food and Drug Administration Good Laboratory Practices regulations. Methodology in the following areas is emphasized: procedures for stock colony maintenance and test insect handling, phenotypic descriptions of Drosophila melanogaster strains used, feeding exposure methodology for aqueous and non-water soluble materials, use of positive and negative control compounds, SLRL testing procedures, daily work schedule, data handling and statistical analysis of results. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1982
- Accession Number
- ADA112912
Entities
People
- J. T. Fruin
- N. R. Powers
- R. A. Wirtz
Organizations
- Letterman Army Hospital