Determination of Nucleotides by Liquid Chromatograpy with a Phosphorus Sensitive Inductively Coupled Plasma Detector.

Abstract

Quantitative analysis of 100 microliters nucleotide samples is accurately performed by analyzing total phosphorus concentration using an inductively coupled plasma (ICP) system. A single calibration using Na2HPO4 (aq) is useful for all nucleotides. A detection limit of 750 ng of phosphorus is determined with an RSD of 4.5%. High performance liquid chromatography (HPLC) is used to separate mixtures of nucleotides on an anion exchange column using acetate buffers. The ICP is used as a selective detector by observing P(I) emissions at 213.6 nm. A Babington nebulizer which is used to aspirate the high percentage salt solutions with 6% efficiency is described. (Author)

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Dec 14, 1981
Accession Number
ADA113489

Entities

People

  • D. R. Heine
  • M. Bonner Denton

Organizations

  • University of Arizona

Tags

DTIC Thesaurus Topics

  • Chemical Compounds
  • Chemical Synthesis
  • Chemistry
  • Chromatography
  • Detection
  • Detectors
  • Elements
  • Emission
  • Flow Rate
  • Governments
  • Liquid Chromatography
  • Military Research
  • Organic Chemistry
  • Organic Compounds
  • Regression Analysis
  • United States
  • Universities

Readers

  • Analytical Chemistry
  • Parasitology and Pharmacology of Malaria.