Effects of Pollutants on Vertebrate Cells in Vitro.
Abstract
Hydrazine and monomethyl hydrazine were examined with respect to their effects on vertebrate cell cultures. Comparative growth and morphological studies demonstrated that both compounds affect the cell surface. The technique of laser cell surface photobleaching of membrane probes is being developed as an assay of membrane fluidity changes. Considerable effort was devoted to perfecting this method by making numerous measurements on different cell types using two different probes. Cells treated with Hz and MMH exhibited distinct differences from the controls with respect to the % of cells showing lateral surface mobility. Furthermore a distinct difference was observed with respect to the qualitative distribution of the surface probe in control cells versus hydrazine-treated cells. Finally, correlative electron microscopy and electro-physiology demonstrated a marked effect of MMH on cell mitochondria and CA++ ion flux. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1981
- Accession Number
- ADA115429
Entities
People
- Michael W. Berns
Organizations
- United States Fish and Wildlife Service