Mechanisms of Oxygen Toxicity at the Cellular Level.
Abstract
Studies using vitamins, precursors, and intermediates with analysis of carbohydrate, amino acid, lipid, protein, RNA and DNA synthesis and of enzyme activities in suspected pathways support the following events during oxygen intoxication of Escherichia coli. Biosynthesis of several amino acids are inhibited. Within less than 5 minutes valine is limiting for protein synthesis (subsequently 9 other amino acids become limiting). The resultant unloaded tRNA's stimulate the 'stringent response' (production of the powerful inhibitor guanosine tetraphosphate) which completely stops biosynthesis. Some of these sites (including quinolinate phosphoribosyl transferase, required for NAD synthesis) should be relevant to man. The data suggest that the vitamins thiamin pyrophosphate and niacin should be tested as potential protective agents against the toxicity in man of oxygen and of chemicals which produce cellular oxidative stress.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 30, 1982
- Accession Number
- ADA116751
Entities
People
- Olen R. Brown