Genetic Analysis of the Nature of Genes Coding Early Enzymes in the Metabolism of Cresol in JPT3-4, a Derivative Strain of Pseudomonas Aeroginosis J1.

Abstract

A pseudomonas strain, JPT3-4, was developed which was competent on all three cresol isomers (para, ortho, and meta). This organism maintained a transmissable FP type plasmid which could transfer the genes required to metabolize cresol to cresol incompetent strains of related species. The transfer of these plasmids was visualized via gel electrophoresis and the metabolic activity of the recipient strains was followed through the use of an oxygen electrode and spectrophotometric analysis of their metabolites. (Author)

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Document Details

Document Type
Technical Report
Publication Date
Apr 29, 1982
Accession Number
ADA117730

Entities

People

  • Howell D. Cobb Jr.
  • John W. Egan Jr.
  • William E. Olive

Organizations

  • Trinity University

Tags

DTIC Thesaurus Topics

  • Acids
  • Air Force
  • Alcohols
  • Amino Acids
  • Anti-Bacterial Agents
  • Bacteria
  • Benzoic Acids
  • Buffers (Chemistry)
  • Chemistry
  • Computer Programs
  • Deoxyribonucleic Acids
  • Engineering
  • Gel Electrophoresis
  • Genetics
  • Oxygen Electrodes
  • Waste Products
  • Waste Water

Fields of Study

  • Biology

Readers

  • Analytical Chemistry
  • Microbial Pathology
  • Molecular Genetics

Technology Areas

  • Biotechnology