In Vitro Studies of Neurotoxic Substances: The Effect of Organophosphates and Acrylamides.
Abstract
The toxicity of acrylamide, n-methylacrylamide, and crotonamide as well as the organophosphates mipafox, leptophos, paraoxon, EPN, OMPA and DFP were studied in order to see whether or not in vivo-in vitro neurotoxicity correlations could be established. The in vitro systems employed were the mouse neuroblastoma cell line NIE-115 and the chick brain, either as cell aggregate cultures or organ culture. In both the neuroblastoma cell culture and chick brain cell/organ culture systems, acrylamide was the most toxic. The ranking of acrylamide, n-methylacrylamide and crotonamide paralleled the ranking reported in vivo. The end-points which showed this ranking included cell viability and neuron-specific enolase activity and aggregate formation by dissociated brain cells. The organophosphate studies emphasized their effect on neurotoxic esterase activity. A model in vitro test has been developed for the evaluation of neurotoxic esterase effects. The test is based on the hen brain assay test developed by Johnson,as a predictor of delayed neuropathy. (AUTHOR)
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 14, 1982
- Accession Number
- ADA119217
Entities
People
- Albert Fedalei
- David Krause
- Jack Spiegel
- Rene M. Filipowski
- Roland M. Nardone
Organizations
- The Catholic University of America