Studies in a Rat Lung Tumor Model: Cellular Biochemistry and Cytogenetics
Abstract
An animal lung tumor model employing intratracheal inoculation of rats with a suspension of 3-methylcholanthrene (MCA) has been used to study induction of 3H-benzo(a)pyrene (3H-BaP) metabolism in lung and liver, and chromosome damage in lung cells. A single inoculation with 1.0 mg MCA increased the enzyme levels in both lung and liver. The enzyme activity in the lung remained above control levels for 8 weeks, and in the liver for more than 2 weeks. Inoculation with 0.1 mg MCA was less effective in both organs. Sister chromatid exchange (SCE) incidence was measured in primary cultures of lung cells from MCA-treated rats. An increased SCE incidence was seen 48 hr posttreatment which persisted for at least 6 weeks after a single treatment with 1.0 mg MCA. Tests for induction of DNA repair synthesis in vivo by MCA (1.0 mg) did not detect evidence of DNA repair.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1982
- Accession Number
- ADA120008
Entities
People
- Ronald E. Rasmussen
Organizations
- University of California, Irvine