The Mutagenicity of Safrole in Salmonella typhimurium, Saccharomyces cerevisiae, and L5178Y Mouse Lymphoma Cells Using the Fluctuation Test.
Abstract
Safrole mutagenicity was tested using bacteria (Salmonella typhimurium), yeast (Saccharomyces cerevisiae D3) and L5178Y mouse lymphoma cells. The fluctuation method was used in this research and is regarded as 50-100 times more sensitive than conventional methods for detecting mutagenicity. In addition, the effect of the coenzyme for sulfation-3'-phosphoadenosine-5' -phosphosulfate (PAPS)-on mutagenicity was evaluated in the bacteria and yeast. Safrole was mutagenic in S. typhimurium strain TA100 at concentrations of 10 and 25 micrograms/ml. PAPS increased mutation frequency at these safrole concentrations. In yeast these same concentrations were mutagenic, but the addition of PAPS was without additional effect. Safrole was not mutagenic for mouse L5178Y cells with ouabain as the selective agent.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1982
- Accession Number
- ADA121019
Entities
People
- Andrea M. Rogers
- Melvin E. Andersen
- P. Leleng To
Organizations
- Air Force Research Laboratory