Cold Injury--Role of Leukocyte Aggregation and Disruption.
Abstract
This Annual Letter Report covers the period November 1, 1981 to November 1, 1982 on the project, Cold injury--Role of Leukocyte Aggregation and Disruption. After prolonged experimentation, a reliable experimental model has been perfected. As noted in the preliminary studies, this amounts to anesthetizing adult New Zealand and White rabbits (body weight, 1500/2000 gms) and administration of Ringer's Lactate in a right ear vein. The left ear is shaved and a thermaprobe connected to a Yellow Springs recorder inserted near the mid-line of the ear 2 1/2 cm from the tip. The ear is marked at centimeter lengths from the tip of the ear to provide accurate level of cold exposure during immersion. The animals are returned to their cages and the ear inspected daily. Quantitative of the injury is now possible by comparing templates of the ear made before injury and seven days after cold exposure. These are traced on heavy cardboard paper and the outline of the frankly necrotic ear traced on the template one week after cold exposure. Weighting the two heavy cardboard templates provides numerical comparison of the area of the ear that has become necrotic due to the cold exposure. This is a highly repeatable figure.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 02, 1982
- Accession Number
- ADA121902
Entities
People
- Ben Eiseman
Organizations
- University of Colorado Health