Localization of Legionella pneumophila in Tissue Using FITC-Conjugated Specific Antibody and a Background Stain

Abstract

Lightly staining formalin-fixed or fresh tissue with Gram's crystal violet obviates interfering nonspecific fluorescence by acting as a metachromatic stain in ultraviolet light. Against the easily recognized background of tissues and cells fluorescein isothiocyanate-tagged Legionella pneumophila antibodies can then identify this bacterium in or on individual cells. This procedure can be run at room temperature in two hours and has the potential for further widespread applicability.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 1982
Accession Number
ADA123108

Entities

People

  • Barbara S. Lowry
  • Filiberto G. Vega Jr.
  • Kenneth W. Hedlund

Organizations

  • United States Army Medical Research Institute of Infectious Diseases

Tags

DTIC Thesaurus Topics

  • Acids
  • Alcohols
  • Animals
  • Antibodies
  • Antigens
  • Bacteria
  • Cells
  • Diseases And Disorders
  • Immune Serums
  • Indicator Dyes
  • Infectious Diseases
  • Laboratory Animals
  • Medical Personnel
  • Microbiology
  • Microorganisms
  • Rodents
  • Tissues

Fields of Study

  • Biology

Readers

  • Chemistry (specifically Chemical Fluorescence)
  • Microbial Pathology
  • Oncology and Biomarker-Based Cancer Detection.