Detection of T-2 Toxin by a Modified Radioimmunoassay.

Abstract

T-2 toxin in serum, urine and saline was analyzed by a modified radioimmuno-assay procedure. The specimens were added directly to the assay tubes without extraction steps. The reaction between antibody and ligands was optimized at one hour. Albumin-coated charcoal was used to separate bound from free radioactivity. Quenching which occurred with hemolyzed specimens was corrected with a wet oxidation process using 60% perchloric acid and 30% hydrogen peroxide. The shorter incubation times resulted in an assay that takes less than 6 h to complete. The average affinity constant of the antibody (km) was 1.75 x (10 to the 10th power) liters/mol. The sensitivity was 1 ng per assay or 10 ng/mi. Among the other trichothecenes tested only H T-2 cross-reacted significantly.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Aug 06, 1982
Accession Number
ADA123195

Entities

People

  • David L. Bunner
  • Fun Sun Chu
  • Joanne Beheler
  • Paulito A. Fontelo

Organizations

  • United States Army Medical Research Institute of Infectious Diseases

Tags

DTIC Thesaurus Topics

  • Albumins
  • Antibodies
  • Detection
  • Fungi
  • Health
  • Immune Serums
  • Inhibition
  • Medical Personnel
  • Mycotoxins
  • Oxidation
  • Perchloric Acid
  • Public Health
  • Radioimmunoassay
  • Rocket Oxidizers
  • Saturation
  • Scintillation
  • Sensitivity

Fields of Study

  • Biology

Readers

  • Analytical Chemistry
  • Mathematics or Statistics
  • Molecular and Cellular Biochemistry