Signal-to-Noise Considerations in Fluctuation Analysis Spectroscopic Techniques (FAST).
Abstract
It is possible to determine fluorescence lifetimes from the frequency response of a fluorophore as well as from its time response. Various schemes for measuring such a response curve are illustrated and include the use of sources whose intensity is modulated at single, swept frequencies, at several frequencies simultaneously, or over a broad, continuous band of frequencies. Detection methods which are considered include a single broad-frequency-response device, one which is swept, and another which hops from one modulated frequency to another. Signal-to-noise expressions are derived for each combination of source and detector and conclusions are drawn from comparison of the S/N expressions. From these considerations and the availability of existing sources for use in time-resolved fluorimetry, the most attractive approaches appear to be one which employs a source modulated at several frequencies simultaneously, each of which is monitored by its own tuned detector or a sinusoidally modulated source, swept synchronously with a tuned detector.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 20, 1983
- Accession Number
- ADA123698
Entities
People
- Gary M. Hieftje
- J. M. Ramsey
Organizations
- Indiana University