Morphogenesis of Dengue Virus. Molecular Biology and Molecular Organization of Proteins.
Abstract
The molecular composition and organization of dengue virus (DV) and Japense encephalitis virus (JEV) components released from infected cells is described in this report. Two forms of infectious particles with different molecular compositions were detected. Polypeptides associated with the two forms are tightly bound to the particles and appear to be integral envelope proteins. JEV and DV infected cells were found to release considerable amounts of viral polypeptides into cell culture medium. Radioimmune precipitation was used to pick out viral polypeptides from a large background of labeled material-Nonimmune separation of polypeptides was attempted using column chromatography. Bifunctional crosslinking reagents have been used to study the molecular organization of flaviviruses. Optimization of crosslinking conditions has lead to the detection of a number of crosslinked polypeptides. Suggested assignments of monomers constituting the crosslinked products is presented. Preliminary results of polypeptide composition analysis of uninfected and infected cell lysates and plasma membrane fractions are presented.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1979
- Accession Number
- ADA124327
Entities
People
- Jarue S. Manning
Organizations
- University of California, Davis