Antibodies from the Lyb-5(-)B Cell Subset Predominate in the Secondary IgG Response to Phosphocholine
Abstract
The X-linked CBA/N immune defect was used to investigate the role of the Lyb-5(-) B cell subset in phosphocholine- (PC) specific memory responses. Immune-defective mice, which express only the LyB-5(-) B cell subset, are unable to mount a primary or secondary T15(+), IgM response to PC but can produce a substantial secondary IgG response. The majority of these IgG anti-PC antibodies are T15(-) and can be inhibited by phenylphosphocholine, but not PC. Normal mice, which possess Lyb-5(+) and Lyb-5(-) B cells, produce both IgM and IgC anti-PC antibodies; however, there is a striking difference in the idiotype and fine specificity of antibodies expressed by these two classes. The IgM anti-PC antibodies are T15(+) and PC-inhibitable, whereas the IgG antibodies are identical to those observed in the immune-defective mice, i.e., T15(-) and PC-noninhibitable. This unexpected difference in both idiotype and fine specificity between IgM and IgG anti-PC antibodies results from activation of different B cell subsets. Lyb-5(+) B cells produce T15(+), PC-inhibitable IgM antibodies, whereas T15(-), PC-noninhibitable IgG antibodies are produced by Lyb-5(-) B cells. These data indicate that a majority of the thymus-dependent, anti-PC IgG memory response arises from Lyb-5(-) B cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1982
- Accession Number
- ADA124493
Entities
People
- Gretchen Guelde
- Irwin Scher
- James J. Kenny
- Linda S. Wicker
Organizations
- Naval Medical Research Center