B Lymphocyte Subpopulation Defined by a Rat Monoclonal Antibody, 14G8
Abstract
14G8 is monoclonal rat antibody that recognizes an antigen found on 30 to 40% of B lymphocytes from normal mice and on approximately 65% of B lymphocytes from mice with the xid-determined immune defect. 14G8(+) B cells from normal mice resemble B cells from mice with the xid-determined defect in that the median amount of membrane IgM expressed per cell is much larger than that of 14G8(-) B cells. The frequency of 14G8(+) cells is highest in neonatal mice (approximately 55% of all spleen cells) and falls with age to approximately 25% of all spleen cells in adult mice. Relatively few lymph node or bone marrow B cells express the antigen recognized by 14G8. 14G8 also reacts with 50% of resident peritoneal cells and with red blood cells. 14G8(+) and 14G8(-) B cell preparations were obtained by fluorescence-activated cell sorting and by adherence to 14G8 coated dishes. 14G8(+) cells responded with in vitro proliferation to both anti-mu and to LPS. Cell cycle analysis indicated that approximately 33% of these cells entered S phase in response to LPS and 38% in response to anti-mu. In contrast, 14G8(-) cells responded poorly to LPS (7% of cells entered S phase) although they showed good responses to anti-mu (40% of cells entered S phase). Thus, 14G8(+) B cells, despite their similarity to B cells from mice with the xid defect, can proliferate to anti-mu, which B cells were defective mice fail to do. 14G8 provides a monoclonal antibody valuable in the description of functional B cell subpopulations.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1982
- Accession Number
- ADA124503
Entities
People
- Aftab Ahmed
- Irwin Scher
- John T. Kung
- Robert Habbersett
- Susan O. Sharrow
- William E. Paul
Organizations
- Naval Medical Research Center